最近，来自歌本哈根大学的科学家利用Videometer公司构建的Radimax发表了题为Semi-field root phenotyping: Root traits for deep nitrate uptake的文章，文章发表于Plant Cell Environ.2021 Nov 21.doi: 10.1111/pce.14227.这已经是利用该设备发表的第五篇文章。该多光谱微根管成像系统专为设施农业设计，也可设计成便携式，应用于常规根系原位表型和生理生态研究。

半田间根系表型：深层硝酸盐吸收的根系特征

摘要

深根冬小麦基因型可以减少硝酸盐淋失，增加氮素吸收。我们旨在研究哪些深根性状与深氮吸收相关，并估计根系性状和深根15N示踪吸收的遗传变异。两年间，冬小麦基因型生长在RadiMax（一个半田间根系筛选设施）中。在主要生长季节进行了三次微根管根成像。开花时，在1.8m深度通过地下滴灌注入15N。对注射区域上方的成熟麦穗进行15N含量分析。根据基于微根管图像的根长数据，构建了82个性状，描述了根的深度、密度、分布和生长情况。用LASSO回归分析了它们预测15N吸收的能力。根系性状预测了两年内示踪物吸收变化分别为24%和14%。根系性状和基因型对示踪剂吸收均有显著影响。2018年，基因型和三个LASSO选择的根系性状预测了示踪剂吸收变化为41%，2019年，基因型和一个根系性状预测的示踪剂吸收变化48%。在这两年中，一个根系性状显著地介导了基因型对示踪剂吸收的影响。来自微根管图像的深根性状可以预测深氮吸收，表明具有培育深氮吸收基因型的潜力。

Semi-field root phenotyping: Root traits for deep nitrate uptake

Tomke Susanne Wacker 1, Olga Popovic 1, Niels Alvin Faircloth Olsen 1, Bo Markussen 2, Abraham George Smith 3, Simon Fiil Svane 1, Kristian Thorup-Kristensen 1

Plant Cell Environ.2021 Nov 21.

doi: 10.1111/pce.14227.

Abstract

Deep rooting winter wheat genotypes can reduce nitrate leaching losses and increase N uptake. We aimed to investigate which deep root traits are correlated to deep N uptake and to estimate genetic variation in root traits and deep 15 N tracer uptake. In two years, winter wheat genotypes were grown in RadiMax, a semi-field root-screening facility. Minirhizotron root imaging was performed three times during the main growing season. At anthesis, 15 N was injected via subsurface drip-irrigation at 1.8m depth. Mature ears from above the injection area were analysed for 15 N content. From minirhizotron image-based root length data, 82 traits were constructed, describing root depth, density, distribution and growth aspects. Their ability to predict 15 N uptake was analysed with LASSO regression. Root traits predicted 24% and 14% of tracer uptake variation in the two years. Both root traits and genotype showed significant effects on tracer uptake. In 2018, genotype and the three LASSO-selected root traits predicted 41% of the variation in tracer uptake, in 2019 genotype and one root trait predicted 48%. In both years, one root trait significantly mediated the genotype effect on tracer uptake. Deep root traits from minirhizotron images can predict deep N uptake, indicating potential to breed deep-N-uptake-genotypes. This article is protected by copyright. All rights reserved.